Biota Molecular Analysis & Bioinformatics Reporting

Our Molecular Techniques

As part of our end-to-end workflow we offer our customers the option of having their samples analysed. Biota Trace have several internal R&D projects working to move the state-of-art from requiring central laboratory processing to in-situ, near real-time molecular analysis.

We work with customers on a project by project basis to provide laboratory screening or analysis or advice on transitioning them to near real-time, in-situ molecular analysis.

Contact us to discuss your project or find out more about in-situ analysis roadmap.

DNA/RNA Extraction & Purification

Our DNA and RNA extraction workflow predominantly focuses on Sterivex filters. We have a range of lysis options for extraction depending on the sample matrix. We use spin-column and bead based purification techniques. We are also able to consider extractions from sediment, soil, plant tissue and scat. We have experience in inhibitor removal and a range of sample extraction characterisation instrumentation (Qubit/Nanodrop).

PCR & Library Preparation

We have experience in primer design alongside the utilisation of published primers, both for single target amplification and for amplicon preparation for subsequent sequencing work. We can conduct initial screening of your samples using gel electrophoresis as an end point or for QC checking prior to sequencing.

qPCR

For single targets / amplicons we are able to provide multiplex (up-to 6 channels) or intercalating dye based quantitative PCR, in single tube or 96 well format.

Next Generation Sequencing

We offer a range of next generation sequencing services. Please contact us to discuss your requirements.

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Our Bioinformatics & Reporting

We offer a range of digital solutions to help our customers derive insights from their data.

Bioinformatics

We continue to invest in the development of bioinformatic pipelines for our common data sets. We are also able to produce bespoke scripts and pipelines for a range of molecular data. We also offer visualisations and graphical representations of complex data which often consists not only of molecular data but data fused with other sensors such as imaging, acoustic, geospatial and metrological.

Reporting

We can work with you to provide data for your scientific, regulatory and compliance reporting requirements. Typical examples include eDNA reporting, Environmental Impact Assessments and Biodiversity Net Gain Analysis.

Dashboard & Data API

Our data can be hosted and made interrogable via APIs so that our customers can integrate into their own software or back-end systems. We offer a dashboard solution, which allows key indicators, as well as raw data in various formats to enable customers to do their own data processing and analysis.